Human Beta-Hydroxybutyric Acid (B-OHB) ELISA Kit

SKU: AE62218HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: N/A

Abbreviation: B-OHB

Alternative Names: N/A

Application: ELISA

Range: 1.23-101 μg/mL

Sensitivity: 0.49 μg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate B-OHB in samples. An antibody specific for B-OHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-OHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-OHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-OHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Beta-Hydroxybutyric acid is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-4-hydroxybutyric acid. Like the other ketone bodies, levels of beta-hydroxybutyrate are raised in ketosis. In humans, beta-hydroxybuty⃀̚⁰̚

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).