Human Aryl Hydrocarbon Receptor, AHR ELISA Kit

SKU: E0664Hu

Size:96 Tests-1 Kit

Background:Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed: 30373764, PubMed: 23275542, PubMed: 7961644, PubMed: 32818467). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed: 30373764, PubMed: 23275542, PubMed: 7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed: 12213388). Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed: 7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed: 7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed: 18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed: 32866000, PubMed: 32818467). Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed: 32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed: 28602820). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed: 28602820). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed: 28602820).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:AHR

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.25ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Aryl Hydrocarbon Receptor

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P35869

MASS(Da):96,147

GeneID:196

Synonyms:Ah receptor;AHR;Aryl hydrocarbon receptor;bHLHe76;Class E basic helix-loop-helix protein 76

Gene names:AHR

Standard solution:2400ng/L