Human Apoptosis-inducing factor 1, mitochondrial,AIFM1 ELISA KIT

SKU: E7258Hu

Size:96 Tests-1 Kit

Background:Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed:20362274, PubMed:23217327, PubMed:17094969). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (By similarity). Binds to DNA in a sequence-independent manner (PubMed:27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed:17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed:19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:26004228). {ECO:0000250|UniProtKB:Q9Z0X1, ECO:0000269|PubMed:17094969, ECO:0000269|PubMed:19418225, ECO:0000269|PubMed:20362274, ECO:0000269|PubMed:23217327, ECO:0000269|PubMed:26004228, ECO:0000269|PubMed:27178839}.; [Isoform 4]: Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. {ECO:0000269|PubMed:16644725}.; [Isoform 5]: Pro-apoptotic isoform. {ECO:0000269|PubMed:16365034}.

Research_area:

Applications:ELISA

Target_protein:AIFM1

Reactivity:Human

Assay type:Sandwich

Sensitivity:20.23ng/L

Detection range:37.5-2400ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O95831

MASS(Da):66901

GeneID:9131

Synonyms:AIFM1 AIF PDCD8

Gene names:AIFM1

Standard solution:4800ng/L