Human Aldo-Keto Reductase Family 1 Member C3, AKR1C3 ELISA Kit

SKU: E3275Hu

Size:96 Tests-1 Kit

Background:Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed:10622721, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:14672942, PubMed:11165022). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed:20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed:15047184, PubMed:20036328, PubMed:10622721, PubMed:11165022, PubMed:10998348, PubMed:19010934). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:10998348, PubMed:14672942, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:10557352). Displays also retinaldehyde reductase activity toward 9-cis-retinal (PubMed:21851338).

Research_area:Signal Transduction

Applications:ELISA

Target_protein:AKR1C3

Reactivity:Human

Assay type:Sandwich

Sensitivity:8.32ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Aldo-keto reductase family 1 member C3

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P42330

MASS(Da):36,853

GeneID:8644

Synonyms:17-beta-HSD 5;17-beta-hydroxysteroid dehydrogenase type 5;3-alpha-HSD type 2;3-alpha-HSD type II, brain;3-alpha-hydroxysteroid dehydrogenase type 2;AKR1C3;Aldo-keto reductase family 1 member C3;Chlordecone reductase homolog HAKRb;DD3;DD-3;DDX;Dihydrodiol dehydrogenase 3;Dihydrodiol dehydrogenase type I;HA1753;HAKRB;HAKRe;hluPGFS;PGFS;Prostaglandin F Synthase;Testosterone 17-beta-dehydrogenase 5

Gene names:AKR1C3

Standard solution:3200ng/L