Human Activin Receptor-Like Kinase 1 (ALK1) ELISA Kit

SKU: AE23729HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P37023

Abbreviation: ACVRL1

Alternative Names: ACVRLK1; ALK-1; ALK1; HHT; HHT2; ORW2; SKR3; TSR-I; TGF-B superfamily receptor type I|activin A receptor; type II-like kinase 1|serine/threonine-protein kinase receptor R3

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate ACVRL1 in samples. An antibody specific for ACVRL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACVRL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACVRL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACVRL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: ACVRL1 encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).