His-tag rabbit pAb

SKU: ES1076

Size: 100μL

Source:Rabbit

Applications:WB;ELISA

Reactivity:Species independent

Dilution:Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

Immunogen:6X His synthetic peptide conjugated to KLH.

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):15kD

Human_gene_id:

Human_swiss_prot_no:

Subcellular_location:

Other_name:6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Background:The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.