HIF1AN Polyclonal Antibody

SKU: E-AB-13556

Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Polyclonal Antibodies

Abbreviation: HIF1AN

Target Synonym: DKFZp762F1811;Factor inhibiting HIF-1;Factor inhibiting HIF1;FIH 1;FIH-1;FIH1;FLJ20615;FLJ22027 ;HIF1AN;HIF1N;Hypoxia inducible factor 1 alpha inhibitor;Hypoxia inducible factor 1 alpha subunit inhibitor;Hypoxia inducible factor asparagine hydroxylase;Hypoxia-inducible factor 1-alpha inhibitor;Hypoxia-inducible factor asparagine hydroxylase;Peptide aspartate beta dioxygenase

Research Areas: Cancer;Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species reactivity: Human;Mouse

Application: WB;IHC

Isotype: IgG

Clonality: Polyclonal

Clone NO.:

UNIProt ID: Q9NWT6

Accession:

Background: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Concentration: 0.6 mg/mL

Immunogen: Synthetic peptide of human HIF1AN

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification method: Affinity purification

Dilution: WB 1:500-1:2000;IHC 1:50-1:300

Calculated MW: 40kDa

ObservedMW: