Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated

SKU: BA1126

Size: 0.5ml

Storage: At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Form:

Reactivity: Mouse

Applications: Flow Cytometry,IF,WB

Application Details: Western blot: 0.2-0.5 μg/mL
Immunofluorescence: 2-10 μg/mL
Flow Cytometry: 2-10 μg/mL

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Specificity: This Fluoro®488 conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human/bovine/rabbit IgG.

Background: Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Immunogen: Mouse IgG (whole molecule).

Clonality: Polyclonal

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Host: Goat

Concentration: 1mg/ml

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