gABA ELISA kit (General species)

SKU: CEA900Ge

Size: 96Tests

# of Times Cited in literature: 29

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: gABA

Target Full Name: Gamma-Aminobutyric Acid

Alternative Names: 4-Aminobutyric Acid

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species gABA / Gamma-Aminobutyric Acid

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 4.94-400pg/mL

Sensitivity: 2.17pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA). No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gamma-Aminobutyric Acid (gABA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gamma-Aminobutyric Acid (gABA) and unlabeled Gamma-Aminobutyric Acid (gABA) (Standards or samples) with the pre-coated antibody specific to Gamma-Aminobutyric Acid (gABA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample.

Research Area: Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;

References Citing This Product: Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum

Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism

Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children

Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension

Sex differences in the glutamate signaling pathway in juvenile rats

A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme

Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission

Differentiation of human glioblastoma U87 cells into cholinergic neuron

Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism

Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.

Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy

Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation

Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases

Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission

Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies