FSTL1 ELISA kit (Human)

SKU: SEJ085Hu

Size: 96Tests

# of Times Cited in literature: 12

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: FSTL1

Target Full Name: Follistatin Like Protein 1

Alternative Names: FRP; FSL1; Follistatin-related protein 1

Target Species: Human

Uniprot: Q12841

Gene ID: 11167

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human FSTL1 / Follistatin Like Protein 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 1.56-100ng/mL

Sensitivity: 0.59ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Follistatin Like Protein 1 (FSTL1). No significant cross-reactivity or interference between Follistatin Like Protein 1 (FSTL1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 1 (FSTL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 1 (FSTL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 1 (FSTL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follistatin Like Protein 1 (FSTL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Reproductive science;

References Citing This Product: Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease.

Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cells

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation

Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary Bypass

Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Men

Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction.

Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortality

High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy state

Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous …

Improved early detection of ovarian cancer using longitudinal multimarker models

Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in mice

Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy

Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease