ERK1/2 Antibody , Cat. # AF0155

SKU: AF0155

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:MAPK3,MAPK1

GeneID:5595,5594

Uniprot:P27361/P28482

Specificity:ERK1/2 Antibody detects endogenous levels of total ERK1/2.

Immunogen:A synthesized peptide derived from human ERK1/2, corresponding to a region within C-terminal amino acids.

Storage:PBS, pH 7.4,50% glycerol.

Reactivity:Human,Mouse,Rat,Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Monkey,Fish

Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit

Application:WB,IHC,IF/ICC,IP,ELISA(peptide)

Dilution:WB: 1:1000-1:5000, IHC: 1:100-1:500, IF/ICC 1:200 IP 1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2833336

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF0155-BP

Des# Blocking Peptide:ERK1/2 Blocking peptide

Blocking Peptide Price:$549 CAD