EasyStep Human AngII(Angiotensin II) ELISA Kit

SKU: ELK098ES

Size:96T

Reactivity:Human

Alternative Names:Ang-II; Angiotensin-2

Assay Type:Competitive Inhibition

Sensitivity:10.63 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Endocrinology;Cardiovascular biology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AngII antigen, and the Human AngII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human AngII to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: