Dog β2-MG(Beta-2-Microglobulin) ELISA Kit

SKU: ELK0336

Size:96T

Reactivity:Dog

Alternative Names:Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin

Assay Type:Sandwich

Sensitivity:0.23 µg/mL

Standard:50 µg/mL

Detection range:0.78-50 µg/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Infection immunity;Kidney biomarker;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P19341