DDDDK-Tag(binds to flag sequnence) rabbit pAb

SKU: ES1078

Size: 100μL

Source:Rabbit

Applications:WB;ELISA;IP;IF

Reactivity:Species independent

Dilution:Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.

Immunogen:DDDDK synthetic peptide conjugated to KLH.

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):

Human_gene_id:

Human_swiss_prot_no:

Subcellular_location:

Other_name:DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Background:The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.