DAO ELISA kit (Human)

SKU: SEJ298Hu

Size: 96Tests

# of Times Cited in literature: 17

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: DAO

Target Full Name: D-Amino Acid Oxidase

Alternative Names: DAMOX

Target Species: Human

Uniprot: P14920

Gene ID: 1610

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human DAO / D-Amino Acid Oxidase

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 1.56-100ng/mL

Sensitivity: 0.59ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO). No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Amino Acid Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Amino Acid Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Enzyme & Kinase;

References Citing This Product: Differential regulation of D-amino acid oxidase (DAO) mRNA and protein expression in human post-mortem brain

Age span expression of schizophrenia susceptibility genes D-amino acid oxidase (DAO) and DAO activator (DAOA) in human post-mortem brain

Expression of D-Amino Acid Oxidase (DAO/DAAO) and D-Amino Acid Oxidase Activator (DAOA/G72) during Development and Aging in the Human Post-mortem Brain.

Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control study

Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and mice

Effect of a chamomile extract in protecting against radiation‐induced intestinal mucositis

Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis

Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response

Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury

The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion

Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity

Psychological and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome Undergoing a Low-FODMAP Diet: The Role of the Intestinal Barrier

Blood D-amino acid oxidase (DAO) levels increased with cognitive decline among people with mild cognitive impairment (MCI): a two-year prospective study