CTXII Mini-sample ELISA kit (Rat)

SKU: MEA686Ra

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: CTXII

Target Full Name: Cross Linked C-Telopeptide Of Type II Collagen

Alternative Names: CTX-II; CTX2

Target Species: Rat

Uniprot: P05539

Gene ID: 25412

Featured Series: ME kit

Featured Series Function: Detects protein (micro-volume samples 15-25 μl)

Specificity: Reactive with Rat CTXII / Cross Linked C-Telopeptide Of Type II Collagen

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 98.8-8,000pg/mL

Sensitivity: 39.4pg/mL

Assay Time: 3h

Sample Size: 15-25uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII). No significant cross-reactivity or interference between Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 25µL standard or sample to each well. And then add 25μL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 25µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.

Research Area: Metabolic pathway;Bone metabolism;Rheumatology;

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