GeneBio Systems

CHEM ELISA kit (Human)

Name: CHEM ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA945Hu
Price: 897.95 EUR
Availability: InStock

SKU:SEA945Hu

€897,95 EUR

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Quantity

Size: 96Tests

# of Times Cited in literature: 24

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CHEM

Target Full Name: Chemerin

Alternative Names: TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

Target Species: Human

Uniprot: Q99969

Gene ID: 5919

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human CHEM / Chemerin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.78-50ng/mL

Sensitivity: 0.29ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM). No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Metabolic pathway;Infection immunity;

References Citing This Product: Serum chemerin and vaspin in non-alcoholic fatty liver disease

Chemerin, vaspin and insulin resistance in chronic hepatitis C

Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats

Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci

Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients

Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects

Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome

Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia

Relationship between plasma chemerin levels and disease severity in COPD patients

Elevated serum chemerin in Chinese women with hyperandrogenic PCOS

Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris