BSA(Bovine Serum Albumin) ELISA Kit

SKU: ELK1042

Size:96T

Reactivity:General

Alternative Names:Bovine Albumin; Fraction V

Assay Type:Competitive Inhibition

Sensitivity:167.8 ng/mL

Standard:30000 ng/mL

Detection range:468.75-30000 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Immune molecule;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BSA in the samples is then determined by comparing the OD of the samples to the standard curve.

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