β-actin Rabbit pAb

SKU: EA014

Size: 100μL

Source:Rabbit

Applications:WB, IHC, IF

Reactivity:Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus

Dilution:WB 1:3,000 IHC 1:200 IF 1:200

Immunogen:Recombinant Protein

Storage_stability:PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality:Polyclonal

Isotype:IgG

Concentration:1mg/mL

Observed_band(KD):43kDa

Human_gene_id:60

Human_swiss_prot_no:P60709

Subcellular_location:Cytoplasm,Cytoskeleton

Other_name:actin antibody, b-actin antibody, beta-actin antibody

Background:β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.