aZGP1 ELISA kit (Human)

SKU: SEL231Hu

Size: 96Tests

# of Times Cited in literature: 9

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: aZGP1

Target Full Name: Alpha-2-Glycoprotein 1, Zinc Binding

Alternative Names: ZA2G; ZAG

Target Species: Human

Uniprot: P25311

Gene ID: 563

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human aZGP1 / Alpha-2-Glycoprotein 1, Zinc Binding

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 4.7-300ng/mL

Sensitivity: 1.8ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Urine, Saliva, Seminal Plasma, Sweat and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). No significant cross-reactivity or interference between Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;

References Citing This Product: Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach

Serum ZAG Levels Were Associated with eGFR Mild Decrease in T2DM Patients with Diabetic Nephropathy.

Quantitative Proteomic Analyses of Tear Fluid in Graves' Orbitopathy

Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in …

Serum Zinc-α2-Glycoprotein Levels in Patients with or without Coronary Artery Disease in Chinese North Population

Low Serum ZAG Levels Correlate With Determinants of the Metabolic Syndrome in Chinese Subjects

Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population

A study on serum pro-neurotensin (PNT), furin, and zinc alpha-2-glycoprotein (ZAG) levels in patients with acromegaly