AP36 ELISA kit (Human)

SKU: CEB865Hu

Size: 96Tests

# of Times Cited in literature: 6

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: AP36

Target Full Name: Apelin 36

Alternative Names: APLN36

Target Species: Human

Uniprot: Q9ULZ1

Gene ID: 8862

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human AP36 / Apelin 36

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 7.41-600pg/mL

Sensitivity: 2.52pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 36 (AP36) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 36 (AP36) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Apelin 36 (AP36). No significant cross-reactivity or interference between Apelin 36 (AP36) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 36 (AP36) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 36 (AP36) and unlabeled Apelin 36 (AP36) (Standards or samples) with the pre-coated antibody specific to Apelin 36 (AP36). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 36 (AP36) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin 36 (AP36) in the sample.

Research Area: Metabolic pathway;Endocrinology;Gastroenterology;

References Citing This Product: Apelin levels in relation with hormonal and metabolic profile in patients with polycystic ovary syndrome

Apelin?levels?in?relation?with?hormonal?and metabolic profile in patients with polycystic ovary syndrome.

Apelin?levels are higher in obese patients with?endometrial?cancer.

Apelin and Atrial Fibrillation: The Role in the Arrhythmia Recurrence Prognosis

Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3

The Relationship between Serum Apelin Levels and the Severity of Calcific Aortic Stenosis

Find the Essence through the Phenomena: Cardiovascular Diseases and Biomarkers