Anti-TXNRD1 Antibody Picoband®

SKU: A01778-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TXNRD1

Specificity:

Background: Thioredoxin reductase 1, cytoplasmic is an enzyme that in humans is encoded by the TXNRD1 gene. The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.

Immunogen: E.coli-derived human TXNRD1 recombinant protein (Position: Y93-Y326).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dammeyer, P., Damdimopoulos, A. E., Nordman, T., Jimenez, A., Miranda-Vizuete, A., Arner, E. S. J. Induction of cell membrane protrusions by the N-terminal glutaredoxin domain of a rare splice variant of human thioredoxin reductase 1. J. Biol. Chem. 283: 2814-2821, 2008. 2. Gasdaska, J. R., Gasdaska, P. Y., Gallegos, A., Powis, G. Human thioredoxin reductase gene localization to chromosomal position 12q23-q24.1 and mRNA distribution in human tissue. Genomics 37: 257-259, 1996. 3. Gasdaska, P. Y., Gasdaska, J. R., Cochran, S., Powis, G. Cloning and sequencing of a human thioredoxin reductase. FEBS Lett. 373: 5-9, 1995.

Uniprot ID: Q16881

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 71 kDa

Gene ID: 7296

Protein Name: Thioredoxin reductase 1, cytoplasmic

Gene Full Name: thioredoxin reductase 1

Synonyms: GRIM 12; GRIM12; KDRF; thioredoxin reductase 1; Thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR; TXNRD1