Anti-Tropomyosin 2/TPM2 Antibody Picoband®

SKU: A03082-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat

Gene Name: TPM2

Specificity:

Background: β-Tropomyosin, also known as tropomyosin beta chain is a protein that in humans is encoded by the TPM2 gene. This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Tropomyosin 2/TPM2, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bamshad, M., Watkins, W. S., Zenger, R. K., Bohnsack, J. F., Carey, J. C., Otterud, B., Krakowiak, P. A., Robertson, M., Jorde, L. B. A gene for distal arthrogryposis type I maps to the pericentromeric region of chromosome 9. Am. J. Hum. Genet. 55: 1153-1158, 1994. 2. Clarke, N. F., Domazetovska, A., Waddell, L., Kornberg, A., McLean, C., North, K. N. Cap disease due to mutation of the beta-tropomyosin gene (TPM2). Neuromusc. Disord. 19: 348-351, 2009. 3. Davidson, A. E., Siddiqui, F. M., Lopez, M. A., Lunt, P., Carlson, H. A., Moore, B. E., Love, S., Born, D. E., Roper, H., Majumdar, A., Jayadev, S., Underhill, H. R., and 14 others. Novel deletion of lysine 7 expands the clinical, histopathological and genetic spectrum of TPM2-related myopathies. Brain 136: 508-521, 2013.

Uniprot ID: P07951

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 64406 MW

Gene ID: 7169

Protein Name: Tropomyosin beta chain

Gene Full Name: tropomyosin 2

Synonyms: AMCD1; Beta tropomyosin; DA1; DA2B; TMSB; TPM2; Tropomyosin 2; tropomyosin 2 (beta); Tropomyosin beta chain