Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 2I3)

SKU: M02197-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: TKT

Specificity:

Background: Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is ly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.

Immunogen: E.coli-derived human Transketolase/TKT recombinant protein (Position: M1-A116).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Abedinia, M., Layfield, R., Jones, S. M., Nixon, P. F., Mattick, J. S. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem. Biophys. Res. Commun. 183: 1159-1166, 1992. 2. Boyle, L., Wamelink, M. M. C., Salomons, G. S., Roos, B., Pop, A., Dauber, A., Hwa, V., Andrew, M., Douglas, J., Feingold, M., Kramer, N., Saitta, S., Retterer, K., Cho, M. T., Begtrup, A., Monaghan, K. G., Wynn, J., Chung, W. K. Mutations in TKT are the cause of a syndrome, including short stature, developmental delay, and congenital heart defects. Am. J. Hum. Genet. 98: 1235-1242, 2016. 3. Coy, J. F., Dubel, S., Kioschis, P., Thomas, K., Micklem, G., Delius, H., Poustka, A. Molecular cloning of tissue-specific transcripts of a transketolase-related gene: implications for the evolution of new vertebrate genes. Genomics 32: 309-316, 1996.

Uniprot ID: P29401

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2I3

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight:

Gene ID: 7086

Protein Name: Transketolase

Gene Full Name: transketolase

Synonyms: TK; TKT; TKT1; transketolase