Anti-TRAM1 Antibody Picoband®

SKU: PA2010

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,IHC-F,ICC,WB

Application Details: Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: TRAM1

Specificity: No cross reactivity with other proteins.

Background: TRAM1 (Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al. (1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gorlich, D., Hartmann, E., Prehn, S., Rapoport, T. A. A protein of the endoplasmic reticulum involved early in polypeptide translocation. Nature 357: 47-52, 1992.

Uniprot ID: Q15629

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 56 kDa

Calculated Molecular Weight: 43072 MW

Gene ID: 23471

Protein Name: Translocating chain-associated membrane protein 1

Gene Full Name: translocation associated membrane protein 1

Synonyms: PNAS 8; PRO1292; TRAM; TRAM1; TRAMP