Anti-TRAIL/TNFSF10 Antibody Picoband®

SKU: A00466-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat

Gene Name: TNFSF10

Specificity: No cross reactivity with other proteins.

Background: TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). It is a member of Tumor Necrosis Factor Ligand Superfamily. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL has got 281 amino acid proteins. TRAIL mRNA is expressed at significant levels in most normal tissues. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) and the receptors DcR1 and DcR2, leading to activation of specific kinases and transcription of genes.

Immunogen: E. coli-derived human TRAIL recombinant protein (Position: N40-A272).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A (May 1996). Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 271 (22): 12687–90. 2. Song JJ, Lee YJ (May 2008). Differential cleavage of Mst1 by caspase-7/-3 is responsible for TRAIL-induced activation of the MAPK superfamily. Cell. Signal. 20 (5): 892–906. 3. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA (December 1995). Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 3 (6): 673–82.

Uniprot ID: P50591

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 33477 MW

Gene ID: 8743

Protein Name: Tumor necrosis factor ligand superfamily member 10

Gene Full Name: TNF superfamily member 10

Synonyms: Apo 2 ligand; Apo 2L; APO2L; CD253; Protein TRAIL; TL2; TNFSF10; TRAIL