Anti-Tlr4 Antibody Picoband®

SKU: A00017-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse

Applications: WB,ELISA

Application Details: Western blot, 0.5-1 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Tlr4

Specificity:

Background: Toll-like receptor 4 is a protein that in humans is encoded by the TLR4 gene. This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.

Immunogen: E.coli-derived mouse Tlr4 recombinant protein (Position: N26-E827).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aderem, A., Ulevitch, R. J. Toll-like receptors in the induction of the innate immune response. Nature 406: 782-787, 2000. 2. Andonegui, G., Bonder, C. S., Green, F., Mullaly, S. C., Zbytnuik, L., Raharjo, E., Kubes, P. Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs. J. Clin. Invest. 111: 1011-1020, 2003. Note: Erratum: J. Clin. Invest. 112: 1264 only, 2003. 3. Andonegui, G., Zhou, H., Bullard, D., Kelly, M. M., Mullaly, S. C., McDonald, B., Long, E. M., Robbins, S. M., Kubes, P. Mice that exclusively express TLR4 on endothelial cells can efficiently clear a lethal systemic gram-negative bacterial infection. J. Clin. Invest. 119: 1921-1930, 2009.

Uniprot ID: Q9QUK6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 96 kDa

Calculated Molecular Weight: 137910 MW

Gene ID: 21898

Protein Name: Toll-like receptor 4

Gene Full Name: toll-like receptor 4

Synonyms: ARMD10; CD284; hToll; TLR4; TOLL; toll like receptor 4