Anti-TLR3 Antibody Picoband®

SKU: A00197

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,WB

Application Details: Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: TLR3

Specificity: No cross reactivity with other proteins.

Background: TLR3 (Toll-like receptor 3) also known as CD283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene is mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-κB and the production of type I interferons. It may thus play a role in host defense against viruses. TLR3 recognizes double-stranded RNA, a form of genetic information carried by some viruses such as retroviruses. Upon recognition, TLR3 induces the activation of NF-κB to increase production of type I interferons which signal other cells to increase their antiviral defenses. Double-stranded RNA is also recognised by the cytoplasmic receptors RIG-I and MDA-5. TLR3 displays a protective role in mouse models of atherosclerosis.[4] In addition, TLR3 activators show effects on human vascular cells.

Immunogen: E. coli-derived human TLR3 recombinant protein (Position: F502-D648).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Edwards, A. O., Swaroop, A., Seddon, J. M.Geographic atrophy in age-related macular degeneration and TLR3. (Letter)New Eng. J. Med. 360: 2254-2255, 2009. 2. Muzio, M., Bosisio, D., Polentarutti, N., D'amico, G., Stoppacciaro, A., Mancinelli, R., van't Veer, C., Penton-Rol, G., Ruco, L. P., Allavena, P., Mantovani, A.Differential expression and regulation of Toll-like receptors (TLR) in human leukocytes: selective expression of TLR3 in dendritic cells.J. Immun. 164: 5998-6004, 2000. 3. Schulz, O., Diebold, S. S., Chen, M., Naslund, T. I., Nolte, M. A., Alexopoulou, L., Azuma, Y.-T., Flavell, R. A., Liljestrom, P., Sousa, C. R.Toll-like receptor 3 promotes cross-priming to virus-infected cells.Nature 433: 887-892, 2005.

Uniprot ID: O15455

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight:

Gene ID: 7098

Protein Name: Toll-like receptor 3

Gene Full Name: toll like receptor 3

Synonyms: CD283; TLR3; toll like receptor 3