Anti-SPA17 Antibody Picoband®

SKU: A07969

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPA17

Specificity:

Background: Sperm surface protein Sp17 is a protein that in humans is encoded by the SPA17 gene. This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Immunogen: E.coli-derived human SPA17 recombinant protein (Position: M1-K136). Human SPA17 shares 75.7% amino acid (aa) sequence identity with mouse SPA17.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Buchli, R., De Jong, A., Robbins, R. L. Genomic organization of an intron-containing sperm protein 17 gene (Sp17-1) and an intronless pseudogene (Sp17-2) in humans: a new model. Biochim. Biophys. Acta 1578: 29-42, 2002. 2. De Jong, A., Buchli, R., Robbins, D. Characterization of sperm protein 17 in human somatic and neoplastic tissue Cancer Lett. 186: 201-209, 2002. 3. Lea, I. A., Richardson, R. T., Widgren, E. E., O'Rand, M. G. Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17. Biochim. Biophys. Acta 1307: 263-266, 1996.

Uniprot ID: Q15506

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 22 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 53340

Protein Name: Sperm surface protein Sp17

Gene Full Name: sperm autoantigenic protein 17

Synonyms: Cancer/testis antigen 22; CT22; SP17; SP17 1; SPA17; sperm autoantigenic protein 17; Sperm protein 17; Sperm surface protein Sp17