Anti-SOCS3 Antibody Picoband®

SKU: PA1707

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse

Gene Name: SOCS3

Specificity: No cross reactivity with other proteins.

Background: SOCS3 (Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOCS3, identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Babon, J. J., McManus, E. J., Yao, S., DeSouza, D. P., Mielke, L. A., Sprigg, N. S., Willson, T. A., Hilton, D. J., Nicola, N. A., Baca, M., Nicholson, S. E., Norton, R. S.The structure of SOCS3 reveals the basis of the extended SH2 domain function and identifies an unstructured insertion that regulates stability.Molec. Cell 22: 205-216, 2006. 2. Chen, Z., Laurence, A., Kanno, Y., Pacher-Zavisin, M., Zhu, B.-M., Tato, C., Yoshimura, A., Hennighausen, L., O'Shea, J. J.Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells.Proc. Nat. Acad. Sci. 103: 8137-8142, 2006. 3. Jo, D., Liu, D., Yao, S., Collins, R. D., Hawiger, J.Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis.Nature Med. 11: 892-898, 2005.

Uniprot ID: O14543

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 30 kDa

Calculated Molecular Weight: 24770 MW

Gene ID: 9021

Protein Name: Suppressor of cytokine signaling 3

Gene Full Name: suppressor of cytokine signaling 3

Synonyms: ATOD4; CIS 3; CIS3; Cish3; SOCS 3; SOCS3; SOCS-3; SSI 3; SSI3; STAT induced STAT inhibitor 3