Anti-SMARCA2/BRM Antibody Picoband®

SKU: A01888-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SMARCA2

Specificity:

Background: Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Immunogen: E.coli-derived human SMARCA2/BRM recombinant protein (Position: V53-K1405).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. de la Serna, I. L., Carlson, K. A., Imbalzano, A. N. Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation. Nature Genet. 27: 187-190, 2001. 2. Hakimi, M.-A., Bochar, D. A., Schmiesing, J. A., Dong, Y., Barak, O. G., Speicher, D. W., Yokomori, K., Shiekhattar, R. A chromatin remodelling complex that loads cohesin onto human chromosomes. Nature 418: 994-998, 2002.

Uniprot ID: P51531

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 210 kDa

Calculated Molecular Weight: 146424 MW

Gene ID: 6595

Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2

Gene Full Name: SWI/SNF related BAF chromatin remodeling complex subunit ATPase 2

Synonyms: ATP dependent helicase SMARCA2; BAF190; BAF190B; BRG1 associated factor 190B; BRM; hBRM; hSNF2a; Protein brahma homolog; SMARCA2; SMARCA2-Specific; SNF2; SNF2 alpha; SNF2A; SNF2L2; SNF2LA; Sth1p; SWI2