Anti-SASS6 Antibody Picoband®

SKU: A05762-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SASS6

Specificity:

Background: Spindle assembly abnormal protein 6 homolog (SAS-6) is a protein that in humans is encoded by the SASS6 gene. The protein encoded by this gene is a central component of centrioles and is necessary for their duplication and function. Centrioles adopt a cartwheel-shaped structure, with the encoded protein forming the hub and spokes inside a microtubule cylinder. Defects in this gene are a cause of autosomal recessive primary microcephaly.

Immunogen: E.coli-derived human SASS6 recombinant protein (Position: E101-S657). Human SASS6 shares 83.7% amino acid (aa) sequence identity with mouse SASS6.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/20/2005. 2. Khan, M. A., Rupp, V. M., Orpinell, M., Hussain, M. S., Altmuller, J., Steinmetz, M. O., Enzinger, C., Thiele, H., Hohne, W., Nurnberg, G., Baig, S. M., Ansar, M., Nurnberg, P., Vincent, J. B., Speicher, M. R., Gonczy, P., Windpassinger, C. A missense mutation in the PISA domain of HsSAS-6 causes autosomal recessive primary microcephaly in a large consanguineous Pakistani family. Hum. Molec. Genet. 23: 5940-5949, 2014. 3. Kleylein-Sohn, J., Westendorf, J., Le Clech, M., Habedanck, R., Stierhof, Y.-D., Nigg, E. A. Plk4-induced centriole biogenesis in human cells. Dev. Cell 13: 190-202, 2007.

Uniprot ID: Q6UVJ0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 74,70-74 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 163786

Protein Name: Spindle assembly abnormal protein 6 homolog

Gene Full Name: SAS-6 centriolar assembly protein

Synonyms: HsSAS 6; SAS 6; SAS6; SASS6