Anti-SART3 Antibody Picoband®

SKU: A03973-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SART3

Specificity:

Background: Squamous cell carcinoma antigen recognized by T-cells 3 is a protein that in humans is encoded by the SART3 gene. The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Immunogen: E.coli-derived human SART3 recombinant protein (Position: D155-A892).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bell, M., Schreiner, S., Damianov, A., Reddy, R., Bindereif, A. p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO J. 21: 2724-2735, 2002. 2. De Troyer, L., Zhao, P., Pastor, T., Baietti, M. F., Barra, J., Vendramin, R., Dok, R., Lechat, B., Najm, P., Van Haver, D., Impens, F., Leucci, E., Sablina, A. A. Stress-induced lncRNA LASTR fosters cancer cell fitness by regulating the activity of the U4/U6 recycling factor SART3. Nucleic Acids Res. 48: 2502-2517, 2020. Note: Erratum. Nucleic Acids Res. 48: 5198-5199, 2020. 3. Frank, J., van Geel, M., van Steensel, M. A. M. Loss of heterozygosity studies on chromosome 12q in disseminated superficial actinic porokeratosis: lessons to be learned. (Letter) J. Invest. Derm. 127: 2058-2059, 2007.

Uniprot ID: Q15020

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 9733

Protein Name: Spliceosome associated factor 3, U4/U6 recycling protein

Gene Full Name: spliceosome associated factor 3, U4/U6 recycling protein

Synonyms: DSAP1; hSART 3; KIAA0156; P100; p110; p110(nrb); RP11 13G14; SART 3; SART3; TIP110