Anti-RNF34 Antibody Picoband®

SKU: A08108-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RNF34

Specificity:

Background: E3 ubiquitin-protein ligase RNF34 is an enzyme that in humans is encoded by the RNF34 gene. The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human RNF34 recombinant protein (Position: E46-S372).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Sasaki, S., Nakamura, T., Arakawa, H., Mori, M., Watanabe, T., Nagawa, H., Croce, C. M. Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer. Oncogene 21: 5024-5030, 2002.

Uniprot ID: Q969K3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight: 17136 MW

Gene ID: 80196

Protein Name: E3 ubiquitin-protein ligase RNF34

Gene Full Name: ring finger protein 34

Synonyms: CARP 1; Caspase regulator CARP1; FYVE RING finger protein Momo; hRFI; RFI; RIF; RIFF; ring finger protein 34; RING finger protein RIFF; RNF34