Anti-Renin receptor/ATP6AP2 Antibody Picoband®

SKU: A02665-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,IP,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: ATP6AP2

Specificity:

Background: The renin receptor also known as ATPase H(+)-transporting lysosomal accessory protein 2, or the prorenin receptor, is a protein that in humans is encoded by the ATP6AP2 gene. This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Renin receptor/ATP6AP2, which shares 100% and 95.8% amino acid (aa) sequence identity with mouse and rat Renin receptor/ATP6AP2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cruciat, C.-M., Ohkawara, B., Acebron, S. P., Karaulanov, E., Reinhard, C., Ingelfinger, D., Boutros, M., Niehrs, C. Requirement of prorenin receptor and vacuolar H(+)-ATPase-mediated acidification for Wnt signaling. Science 327: 459-463, 2010. 2. Demirci, F. Y. K., White, N. J., Rigatti, B. W., Lewis, K. F., Gorin, M. B. Identification, genomic structure, and screening of the vacuolar proton-ATPase membrane sector-associated protein M8-9 gene within the COD1 critical region (Xp11.4). Molec. Vis. 7: 234-239, 2001. Note: Electronic Article. 3. Dubos, A., Castells-Nobau, A., Meziane, H., Oortveld, M. A. W., Houbaert, X., Iacono, G., Martin, C., Mittelhaeuser, C., Lalanne, V., Kramer, J. M., Bhukel, A., Quentin, C., and 10 others. Conditional deletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration. Hum. Molec. Genet. 24: 6736-6755, 2015.

Uniprot ID: O75787

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 49 kDa

Calculated Molecular Weight: 39 kDa

Gene ID: 10159

Protein Name: Renin receptor

Gene Full Name: ATPase H+ transporting accessory protein 2

Synonyms: ATP6AP2, Renin receptor, APT6M8 9, ATPase H(+)-transporting lysosomal accessory protein 2, ATP6M8-9