Anti-RED1/ADARB1 Antibody Picoband®

SKU: A01810-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ADARB1

Specificity:

Background: Double-stranded RNA-specific editase 1 is an enzyme that in humans is encoded by the ADARB1 gene. This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region.

Immunogen: E.coli-derived human RED1/ADARB1 recombinant protein (Position: K234-R714).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Higuchi, M., Maas, S., Single, F. N., Hartner, J., Rozov, A., Burnashev, N., Feldmeyer, D., Sprengel, R., Seeburg, P. H. Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2. Nature 406: 78-81, 2000. 2. Lai, F., Chen, C.-X., Carter, K. C., Nishikura, K. Editing of glutamate receptor B subunit ion channel RNAs by four alternatively spliced DRADA2 double-stranded RNA adenosine deaminases. Molec. Cell. Biol. 17: 2413-2424, 1997. 3. Macbeth, M. R., Schubert, H. L., VanDemark, A. P., Lingam, A. T., Hill, C. P., Bass, B. L. Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing. Science 309: 1534-1539, 2005.

Uniprot ID: P78563

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight:

Gene ID: 104

Protein Name: Double-stranded RNA-specific editase 1

Gene Full Name: adenosine deaminase RNA specific B1

Synonyms: ADAR2; ADAR2a; ADAR2a L1; ADAR2a L2; ADAR2a L3; ADAR2b; ADAR2c; ADAR2d; ADAR2g; ADARB1; DRABA2; DRADA2; dsRNA adenosine deaminase; RED1; RNA editing deaminase 1; RNA editing enzyme 1