Anti-PUS7 Antibody Picoband®

SKU: A13434-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
mmunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PUS7

Specificity:

Background: Enables enzyme binding activity and pseudouridine synthase activity. Involved in several processes, including pseudouridine synthesis; regulation of hematopoietic stem cell differentiation; and regulation of mesoderm development. Located in nucleus.

Immunogen: E.coli-derived human PUS7 recombinant protein (Position: D294-H541).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. de Brouwer, A. P. M., Abou Jamra, R., Kortel, N., Soyris, C., Polla D. L., Safra, M., Zisso, A., Powell, C. A., Rebelo-Guiomar, P., Dinges, N., Morin, V., Stock, M., and 16 others. Variants in PUS7 cause intellectual disability with speech delay, microcephaly, short stature, and aggressive behavior. Am. J. Hum. Genet. 103: 1045-1052, 2018. 2. Han, S. T., Kim, A. C., Garcia, K., Schimmenti, L. A., Macnamara, E., Undiagnosed Diseases Network, Gahl, W. A., Malicdan, M. C., Tifft, C. J. PUS7 deficiency in human patients causes profound neurodevelopmental phenotype by dysregulating protein translation. Molec. Genet. Metab. 135: 221-229, 2022. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/10/2015.

Uniprot ID: Q96PZ0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 75 kDa

Gene ID: 54517

Protein Name: Pseudouridylate synthase 7 homolog

Gene Full Name: pseudouridine synthase 7

Synonyms: PUS7; KIAA1897; Pseudouridylate synthase 7 homolog