Anti-PUF-A/KIAA0020/PUM3 Antibody Picoband®

SKU: A11257-4

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PUM3

Specificity:

Background: Enables RNA binding activity. Predicted to be involved in regulation of translation. Located in chromosome; endoplasmic reticulum; and nuclear lumen.

Immunogen: E.coli-derived human PUF-A/KIAA0020/PUM3 recombinant protein (Position: Q318-K631). Human PUF-A/KIAA0020/PUM3 shares 91.7% and 90.1% amino acid (aa) sequence identity with mouse and rat PUF-A/KIAA0020/PUM3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Brickner, A. G., Warren, E. H., Caldwell, J. A., Akatsuka, Y., Golovina, T. N., Zarling, A. L., Shabanowitz, J., Eisenlohr, L. C., Hunt, D. F., Engelhard, V. H., Riddell, S. R. The immunogenicity of a new human minor histocompatibility antigen results from differential antigen processing. J. Exp. Med. 193: 195-205, 2001. 2. Nomura, N., Miyajima, N., Sazuka, T., Tanaka, A., Kawarabayashi, Y., Sato, S., Nagase, T., Seki, N., Ishikawa, K., Tabata, S. Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line, KG-1. DNA Res. 1: 27-35, 1994. Note: Erratum: DNA Res. 2: 210 only, 1995. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 02/25/2020.

Uniprot ID: Q15397

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 74 kDa

Gene ID: 9933

Protein Name: Pumilio homolog 3

Gene Full Name: pumilio RNA binding family member 3

Synonyms: KIAA0020, PUM3, Minor histocompatibility antigen HA-8, HLA-HA8, HBV X-transactivated gene 5 protein