Anti-PU.1/SPI1 Antibody Picoband®

SKU: A01116-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPI1

Specificity:

Background: Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human PU.1/SPI1 recombinant protein (Position: M1-R212).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1. 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998). The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS. J. Biol. Chem. 273 (9): 4838–42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. Oncogene 5 (5): 663–8.

Uniprot ID: P17947

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight:

Gene ID: 6688

Protein Name: Transcription factor PU.1

Gene Full Name: Spi-1 proto-oncogene

Synonyms: 31 kDa transforming protein; OF; PU.1; SFPI1; SPI 1; SPI A; SPI1; Transcription factor PU.1