Anti-PRTN3 Antibody Picoband®

SKU: A03863-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRTN3

Specificity:

Background: Proteinase 3, also known as PRTN3, is an enzyme that in humans is encoded by the PRTN3 gene. Enables enzyme binding activity; serine-type endopeptidase activity; and signaling receptor binding activity. Involved in several processes, including mature conventional dendritic cell differentiation; membrane protein ectodomain proteolysis; and neutrophil extravasation. Located in azurophil granule lumen; cytosol; and plasma membrane raft. Colocalizes with plasma membrane.

Immunogen: E.coli-derived human PRTN3 recombinant protein (Position: A23-D241).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Belaaouaj, A., Walsh, B. C., Jenkins, N. A., Copeland, N. G., Shapiro, S. D. Characterization of the mouse neutrophil elastase gene and localization to chromosome 10. Mammalian Genome 8: 5-8, 1997. 2. Campanelli, D., Melchior, M., Fu, Y., Nakata, M., Shuman, H., Nathan, C., Gabay, J. E. Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils. J. Exp. Med. 172: 1709-1715, 1990. 3. Davies, D. J., Moran, J. E., Niall, J. F., Ryan, G. B. Segmental necrotising glomerulonephritis with antineutrophil antibody: possible arbovirus aetiology. Brit. Med. J. 285: 606 only, 1982.

Uniprot ID: P24158

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 32 kDa

Calculated Molecular Weight: 28 kDa

Gene ID: 5657

Protein Name: Myeloblastin

Gene Full Name: proteinase 3

Synonyms: ACPA; AGP7; C ANCA; C ANCA antigen; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; P29; PR 3; PR3; proteinase 3; PRTN3; Wegener autoantigen