Anti-Profilin 2/PFN2 Antibody Picoband®

SKU: PA2162

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunofluorescence, 5μg/ml, Human

Gene Name: PFN2

Specificity: No cross reactivity with other proteins.

Background: Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gieselmann, R., Kwiatkowski, D. J., Janmey, P. A., Witke, W. Distinct biochemical characteristics of the two human profilin isoforms. Europ. J. Biochem. 229: 621-628, 1995. 2. Joensuu, T., Blanco, G., Pakarinen, L., Sistonen, P., Kaariainen, H., Brown, S., de la Chapelle, A., Sankila, E.-M. Refined mapping of the Usher syndrome type III locus on chromosome 3, exclusion of candidate genes, and identification of the putative mouse homologous region. Genomics 38: 255-263, 1996.

Uniprot ID: P35080

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight: 15 kDa

Gene ID: 5217

Protein Name: Profilin-2

Gene Full Name: profilin 2

Synonyms: D3S1319E; PFL; PFN2; Prifilin 2; PROF2; profilin 2; Profilin II