Anti-PRMT5 Antibody Picoband®

SKU: A00635-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRMT5

Specificity:

Background: Protein arginine N-methyltransferase 5is anenzymethat in humans is encoded by thePRMT5gene. It is mapped to 14q11.2. This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human PRMT5 recombinant protein (Position: S16-D355).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gilbreth M, Yang P, Bartholomeusz G, Pimental RA, Kansra S, Gadiraju R, Marcus S (Jan 1999). Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. Proc Natl Acad Sci U S A. 95 (25): 14781–6. 2. Kim S, Gunesdogan, U, Zylicz JJ, Hackett, JA, Cougot, D, Bao, S, Lee, C, Dietmann, S, Allen, GE, Sngupta, R, Surani MA (Nov 2014). PRMT5 Protects Genomic Integrity during Global DNA Demethylation in Primordial Germ Cells and Preimplantation Embryos. Molecular Cell. 56 (4): 564–579. 3. Friesen WJ, Wyce A, Paushkin S, Abel L, Rappsilber J, Mann M, Dreyfuss G (Mar 2002). A novel WD repeat protein component of the methylosome binds Sm proteins. J. Biol. Chem. 277 (10): 8243–7.

Uniprot ID: O14744

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight:

Gene ID: 10419

Protein Name: Protein arginine N-methyltransferase 5

Gene Full Name: protein arginine methyltransferase 5

Synonyms: 72 kDa ICln binding protein; HRMT1L5; IBP72; Jak binding protein 1; JBP1; PRMT5; SKB1; SKB1 homolog; SKB1Hs