Anti-PPP2R5B Antibody Picoband®

SKU: A09894-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PPP2R5B

Specificity:

Background: Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform is an enzyme that in humans is encoded by the PPP2R5B gene. The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily.

Immunogen: E.coli-derived human PPP2R5B recombinant protein (Position: Q56-A492).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Boyle, M. I., Jespersgaard, C., Nazaryan, L., Ravn, K., Brondum-Nielsen, K., Bisgaard, A.-M., Tumer, Z. Deletion of 11q12.3-11q13.1 in a patient with intellectual disability and childhood facial features resembling Cornelia de Lange syndrome. Gene 572: 130-134, 2015. 2. European Consortium on MEN1. Mapping of the gene encoding the B56-beta subunit of protein phosphatase 2A (PPP2R5B) to a 0.5-Mb region of chromosome 11q13 and its exclusion as a candidate gene for multiple endocrine neoplasia type 1 (MEN1). Hum. Genet. 100: 481-485, 1997. 3. Loveday, C., Tatton-Brown, K., Clarke, M., Westwood, I., Renwick, A., Ramsay, E., Nemeth, A., Campbell, J., Joss, S., Gardner, M., Zachariou, A., Elliott, A., Ruark, E., van Montfort, R., Childhood Overgrowth Collaboration, Rahman, N. Mutations in the PP2A regulatory subunit B family genes PPP2R5B, PPP2R5C and PPP2R5D cause human overgrowth. Hum. Molec. Genet. 24: 4775-4779, 2015. Note: Erratum: Hum. Molec. Genet. 28: 1578 only, 2019.

Uniprot ID: Q15173

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight:

Gene ID: 5526

Protein Name: Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform

Gene Full Name: protein phosphatase 2 regulatory subunit B'beta

Synonyms: PPP2R5B; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform; PP2A B subunit isoform B'-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta