Anti-PON1 Antibody Picoband® (monoclonal, 9D3)

SKU: M00516

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA (Cap),IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml

Gene Name: Pon1

Specificity: No cross reactivity with other proteins.

Background: Isocitrate dehydrogenase [NADP], mitochondrialis anenzymethat in humans is encoded by theIDH2gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Immunogen: E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Costa LG, Cole TB, Vitalone A, Furlong CE (2005). Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity. CLINICA CHEMICA ACTA 352 (1-2): 37–47. 2. Khateeb J, Gantman A, Kreitenberg AJ, Aviram M, Fuhrman B (January 2010). Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway.Atherosclerosis 208 (1): 119–25. 3. Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN (May 1996). The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics 33 (3): 498–507.

Uniprot ID: P52430

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 9D3

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight:

Gene ID: 18979

Protein Name: Serum paraoxonase/arylesterase 1

Gene Full Name: paraoxonase 1

Synonyms: A esterase 1; Aromatic esterase 1; ESA; K 45; paraoxonase 1; PON; PON 1; PON1; Serum aryldialkylphosphatase 1