Anti-POLR3D Antibody Picoband®

SKU: A11611-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: POLR3D

Specificity:

Background: DNA-ed RNA polymerase III subunit RPC4 is an enzyme that in humans is encoded by the POLR3D gene. This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures.

Immunogen: E.coli-derived human POLR3D recombinant protein (Position: K231-L360). Human POLR3D shares 92.3% amino acid (aa) sequence identity with mouse POLR3D.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Greco, A., Ittmann, M., Barletta, C., Basilico, C., Croce, C. M., Cannizzaro, L. A., Huebner, K. Chromosomal localization of human genes required for G(1) progression in mammalian cells. Genomics 4: 240-245, 1989. 2. Ittmann, M., Greco, A., Basilico, C. Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells. Molec. Cell. Biol. 7: 3386-3393, 1987.

Uniprot ID: P05423

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight:

Gene ID: 661

Protein Name: DNA-directed RNA polymerase III subunit RPC4

Gene Full Name: RNA polymerase III subunit D

Synonyms: POLR3D; BN51; BN51T; DNA-directed RNA polymerase III subunit RPC4; RNA polymerase III subunit C4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog