Anti-PDXK Antibody Picoband®

SKU: A06683-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PDXK

Specificity:

Background: Pyridoxal kinase is an enzyme that in humans is encoded by the PDXK gene. The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Immunogen: E.coli-derived human PDXK recombinant protein (Position: H13-E280). Human PDXK shares 85.4% and 85.1% amino acid (aa) sequence identity with mouse and rat PDXK, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chelban, V., Wilson, M. P., Chardon, J. W., Vandrovcova, J., Zanetti, M. N., Zamba-Papanicolaou, E., Efthymiou, S., Pope, S., Conte, M. R., Abis, G., Liu, Y.-T., Tribollet, E., and 26 others. PDXK mutations cause polyneuropathy responsive to pyridoxal 5-prime-phosphate supplementation. Ann. Neurol. 86: 225-240, 2019. 2. Chern, C. J., Beutler, E. Biochemical and electrophoretic studies of erythrocyte pyridoxine kinase in white and black Americans. Am. J. Hum. Genet. 28: 9-17, 1976. 3. Gachon, F., Fonjallaz, P., Damiola, F., Gos, P., Kodama, T., Zakany, J., Duboule, D., Petit, B., Tafti, M., Schibler, U. The loss of circadian PAR bZip transcription factors results in epilepsy. Genes Dev. 18: 1397-1412, 2004.

Uniprot ID: O00764

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 8566

Protein Name: Pyridoxal kinase

Gene Full Name: pyridoxal kinase

Synonyms: C21orf124; C21orf97; PDXK; PKH; PNK; PRED79; Pyridoxal kinase; Pyridoxine kinase