Anti-Pancreatic Lipase/PNLIP Antibody Picoband®

SKU: A04935-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.5-1 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PNLIP

Specificity:

Background: This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea.

Immunogen: E.coli-derived human Pancreatic Lipase/PNLIP recombinant protein (Position: I36-D404). Human PNLIP shares 78.7% amino acid (aa) sequence identity with mouse and rat PNLIP.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Behar, D. M., Basel-Vanagaite, L., Glaser, F., Kaplan, M., Tzur, S., Magal, N., Eidlitz-Markus, T., Haimi-Cohen, Y., Sarig, G., Bormans, C., Shohat, M., Zeharia, A. Identification of a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency. J. Lipid Res. 55: 307-312, 2014. 2. Davis, R. C., Diep, A., Hunziker, W., Klisak, I., Mohandas, T., Schotz, M. C., Sparkes, R. S., Lusis, A. J. Assignment of human pancreatic lipase gene (PNLIP) to chromosome 10q24-q26. Genomics 11: 1164-1166, 1991. 3. Lowe, M. E., Rosenblum, J. L., Strauss, A. W. Cloning and characterization of human pancreatic lipase cDNA. J. Biol. Chem. 264: 20042-20048, 1989.

Uniprot ID: P16233

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 51 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5406

Protein Name: Pancreatic triacylglycerol lipase

Gene Full Name: pancreatic lipase

Synonyms: Lipase; Pancreatic Lipase; PL; PNLIP; PTL