Anti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1)

SKU: M04136-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: PTGES3

Specificity:

Background: Prostaglandin E synthase 3 (cytosolic)is anenzymethat in humans is encoded by the PTGES3gene. It is mapped to 12q13.3; 12. This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.

Immunogen: E.coli-derived human p23/PTGES3 recombinant protein (Position: M1-K79).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Weaver AJ, Sullivan WP, Felts SJ, et al. (2000). Crystal structure and activity of human p23, a heat shock protein 90 co-chaperone. J. Biol. Chem. 275 (30): 23045–23052. 2. Tanioka T, Nakatani Y, Semmyo N, et al. (2000). Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis. J. Biol. Chem. 275 (42): 32775–32782. 3. Dittmar KD, Pratt WB (1997). Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation. J. Biol. Chem. 272 (20): 13047–13054.

Uniprot ID: Q15185

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 9D3D1

Observed Molecular Weight: 23 kDa

Calculated Molecular Weight:

Gene ID: 10728

Protein Name: Prostaglandin E synthase 3

Gene Full Name: prostaglandin E synthase 3

Synonyms: cPGES; Hsp90 co chaperone; P23; Prostaglandin E synthase 3; PTGES3; TEBP; Telomerase binding protein p23