Anti-NXPH3 Antibody Picoband®

SKU: A14597-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NXPH3

Specificity:

Background: Neurexophilin-3 is a protein that in humans is encoded by the NXPH3 gene. Neurexophilin-3 (NXPH-3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family. The 252 amino acid (aa) NXPH-3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein. Mature rat NXPH-3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH-3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion. NXPH-3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development. NXPH-1 is the neurexophilin most similar to NXPH-3, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to overlap, with NXPH-1 expression occurring mainly by cells that resemble inhibitory interneurons. Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release. Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show specific defects in motor coordination. Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins.

Immunogen: E.coli-derived human NXPH3 recombinant protein (Position: R5-K217). Human NXPH3 shares 95.8% amino acid (aa) sequence identity with mouse and rat NXPH3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 10/24/2014. 2. Missler, M., Sudhof, T. C. Neurexophilins form a conserved family of neuropeptide-like glycoproteins. J. Neurosci. 18: 3630-3638, 1998. 3. Petrenko, A. G., Ullrich, B., Missler, M., Krasnoperov, V., Rosahl, T. W., Sudhof, T. C. Structure and evolution of neurexophilin. J. Neurosci. 16: 4360-4369, 1996.

Uniprot ID: O95157

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight:

Gene ID: 11248

Protein Name: Neurexophilin-3

Gene Full Name: neurexophilin 3

Synonyms: NXPH3; KIAA1159; NPH3; UNQ687/PRO1327; Neurexophilin-3