Anti-Nucleobindin 2/NUCB2 Antibody Picoband®

SKU: PB9876

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse

Gene Name: Nucb2

Specificity: No cross reactivity with other proteins.

Background: Nucleobindin-2?is a?calcium-binding?EF-hand protein?that in humans is encoded by the?NUCB2?gene. By exon trapping and sequence analysis,?the?NUCB2?sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.

Immunogen: E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: NUCB2 nucleobindin 2. 2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (February 1995). Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. Biol Chem Hoppe Seyler 375 (8): 497–512. 3. DeAngelis, M., Drury, S., Doucet, J., Sherry, S., Buckley, L., Pelias, M., Deininger, P., Keats, B., Batzer, M. Construction of a BAC contig and isolation of candidate genes in the Acadian Usher syndrome (USH1C) critical region. (Abstract) Am. J. Hum. Genet. 61 (suppl.): A233 only, 1997.

Uniprot ID: P81117

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 50304 MW

Gene ID: 53322

Protein Name: Nucleobindin-2

Gene Full Name: nucleobindin 2

Synonyms: DNA binding protein NEFA; Gastric cancer antigen Zg4; NEFA; NUCB2; nucleobindin 2